Dataset for Optimized submerged cultivation for γ-aminobutyric acid-enriched sprouts from brown rice

This research aimed to find the optimal condition for cultivating sprouted brown rice.
The following conditions were optimized: cultivation water (distilled water (DW) or slightly acidic electrolyzed water (SAEW) with the addition of effective microorganisms), dark cultivation period, seeding density, water level, and temperature.
The parameters measured were: average length, sprouting ratio, fresh weight, dry weight, and GABA content.

First, the optimal cultivation water was determined by combining DW and SAEW with six levels of EM concentrations (0, 0.250, 0.625, 1.000, 1.375, and 1.750 ml/l).
Then, single factor experiments were performed by changing the dark treatment duration, seeding density, water level, and temperature. Each factor was studied separately while fixing all other factors to the standard condition. The standard condition was as follows: cultivation water of SAEW with an EM concentration of 1.00 ml/l, dark treatment for 24 h, seeding density of 0.150 g/cm², water level of 1.5 cm, and culture temperature of 30 °C. The data for these two experiments are uploaded as files 1_1 and 1_2.
Following the single factor experiments, an orthogonal experiment was performed by changing three factors: temperature, seeding density, and water level. The data for this experiment is uploaded as file 2.
Finally, the GAD level was measured to up to 9 days of cultivation. The data for this experiment is uploaded as file 3.

本研究旨在探寻培育发芽糙米的最佳条件。所优化的条件包括：培育用水（蒸馏水（DW）或略带酸性的电解水（SAEW）中添加有效微生物）、暗培养期、播种密度、水位和温度。测量的参数包括：平均长度、发芽率、鲜重、干重以及γ-氨基丁酸（GABA）含量。首先，通过将DW与SAEW以六种水平的有效微生物浓度（0、0.250、0.625、1.000、1.375和1.750 ml/l）相结合，确定了最佳的培育用水。随后，通过改变暗处理时间、播种密度、水位和温度，进行了单因素实验。在固定其他因素至标准条件下，单独研究了每个因素。标准条件如下：SAEW中有效微生物浓度为1.00 ml/l的培育用水、24小时的暗处理、0.150 g/cm²的播种密度、1.5 cm的水位和30 °C的培养温度。这两组实验的数据分别上传为文件1_1和1_2。在单因素实验之后，通过改变温度、播种密度和水位这三个因素，进行了正交实验。该实验的数据上传为文件2。最后，对γ-氨基丁酸（GABA）含量进行了长达9天的培育期测量。该实验的数据上传为文件3。