Near-isotropic, reconstructed volume electron microscopy (FIB-SEM) of Drosophila mushroom body (jrc_fly-mb-1a)

<b>This acquisition is part of the CellMap 2024 Segmentation Challenge</b><b>Challenge DOI:</b> https://doi.org/10.25378/janelia.c.7456966<b>Challenge Website:</b> https://cellmapchallenge.janelia.org/<b>Sample</b>: Alpha lobe of CantonS G1x w1118 female fly, 6 days old<b>Sample Description</b>: The sample consists of the alpha lobe from a 6-day-old CantonS G1x w1118 female fly. The tissue was prepared using high-pressure freezing and freeze substitution with 1% OsO₄, 0.2% uranyl acetate, and 3% water in acetone, followed by resin embedding in Durcupan. A 235 μm frontal slice was obtained using a vibrating microtome (Leica VT1000) and prefixed in 2.5% glutaraldehyde and 2.5% paraformaldehyde in 0.1M cacodylate buffer for 15 minutes. After embedding, the sample was polymerized, mounted on a copper stub, and trimmed to the alpha lobe region with an ultramicrotome (Leica UC7) for FIB-SEM imaging. The sample was provided by Yoshinori Aso (HHMI/Janelia), prepared for imaging by Zhiyuan Lu (HHMI/Janelia), imaged by Song Pang and C. Shan Xu (HHMI/Janelia), and post-processed and stitched by Eric Trautman and Stephan Preibisch (HHMI/Janelia).<b>Protocol</b>: High-pressure freezing, freeze substitution with 1% OsO₄, 0.2% UA, and 3% H₂O in acetone; resin embedding in Durcupan. Fly tissue was sectioned using a vibrating microtome (Leica VT1000) to obtain a 235 μm frontal slice, prefixed in 2.5% glutaraldehyde and 2.5% paraformaldehyde in 0.1M cacodylate buffer for 15 minutes. After embedding, the sample was polymerized, mounted on a copper stub, and trimmed to the a-lobe region with an ultramicrotome (Leica UC7) for FIB-SEM imaging.<br><br><b>Contributions</b>: Sample provided by Yoshinori Aso (HHMI/Janelia), prepared for imaging by Zhiyuan Lu (HHMI/Janelia), imaged by Song Pang and C. Shan Xu (HHMI/Janelia), and post-processed and stitched by Eric Trautman and Stephan Preibisch (HHMI/Janelia).<b>Acquisition ID</b>: jrc_fly-mb-1a<b>Voxel size (nm)</b>: 4 x 4 x 4 (x, y, z)<br><br><b>Data dimensions (µm)</b>: 44.02 x 37.14 x 38.02 (x, y, z)<b>Imaging start date</b>: 2020-12-20<b>Imaging duration (days)</b>: 38<b>Landing energy (eV)</b>: 700<b>Imaging current (nA)</b>: .25<b>Scanning speed (MHz)</b>: .5<b>Dataset URL</b>: s3://janelia-cosem-datasets/jrc_fly-mb-1a/jrc_fly-mb-1a.zarr/recon-1/em/<b>EM DOI</b>: https://doi.org/10.25378/janelia.27075448<b>Visualization Website</b>: https://openorganelle.janelia.org/datasets/jrc_fly-mb-1a<b>Publication</b>: Xu et al., 2021