PRC1 directs PRC2-H3K27me3 deposition to shield adult spermatogonial stem cells from differentiation [RNA-seq]

To understand the functional role of PRC1 in defining the epigenomic and transcriptional landscape in Aundiff, we eliminated PRC1 function in germ cells using Ddx4-Cre to mutate the E3 ubiquitin ligase RNF2 on a background lacking its partially redundant paralog RING1 (RING1A) to create PRC1 conditional knockout (PRC1cKO) mice. We discover a key role of Polycomb complexes in shielding the undifferentiated state of mouse adult spermatogonia. We then performed gene expression profiling analysis using data obtained from RNA-seq of WT, PRC1ctrl and PRC1cKO spermatogonial cells. Comparative gene expression profiling analysis of RNA-seq data for (1) WT Aundiff and Adiff spermatogonia; (2) PRC1ctrl and PRC1cKO Aundiff spermatogonia; (3) PRC1ctrl and PRC1cKO Aundiff spermatogonia after win18,446 inhibition.