Probing endogenous peptide acceptor substrate specificities of TbSTT3A and TbSTT3B
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https://www.omicsdi.org/dataset/pride/PXD007267
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资源简介:
We performed glycoproteomics on endogenous parasite glycoproteins using sequential endoglycosidase-H and peptide-N-glycosidase-F digestions, the latter in the presence of H2[18O]. This allowed us to assess the relative occupancies of native N-glycosylation sites by endoglycosidase-H resistant N-glycans originating from Man5GlcNAc2-PP-dolichol transferred by TbSTT3A, and endoglycosidase-H sensitive N-glycans originating from Man9GlcNAc2-PP-dolichol transferred by TbSTT3B.
创建时间:
2017-09-20



