Acute Changes in Rat Tissue Gene Expression Following Exposure to Flight Relevant Hypobaria

We exposed healthy, young male rats to different levels of hypobaria (4000 ft or 8000 ft eqiv.) and/or hyperoxia (100% oxygen) for different durations of time (5 or 10 h). We collected heart, lung, brain (hippocampus), and blood tissue samples, processed them for RNA, and performed microarray analysis on the samples.本研究将健康幼年雄性大鼠暴露于不同水平的低气压（hypobaria，等效于4000英尺或8000英尺海拔）和/或高氧（hyperoxia，100%氧气）环境，暴露时长分别为5小时或10小时。研究人员采集了大鼠的心脏、肺、脑（海马体（hippocampus））及血液组织样本，对样本进行RNA提取及相关处理，并开展微阵列分析（microarray analysis）。