Bacillus cereus strain:T8 Genome sequencing. Bacillus cereus strain:T8

Chitooligosaccharide has antifungal, immunomodulatory, antitumor and antioxidant functions.Chitinase is naturally produced by microbes, fungi, plants, insects and animals. Among them, chitinase produced by microbes has received increasing consideration, and potentially fill two needs: they decrease the ecological perils of waste administration, and can increase the worth of the degraded products. Bacillus wiedmannii T8, belonging to Bacillus cereus group, was isolated from from soil samples in Qingdao city,Shandong province, Eastern China in July 2023. A copper-dependent lytic polysaccharide monooxygenase (LPMO) can break the glycosidic bonds of crystalline and/or chitin polysaccharides by oxidation, providing more active sites for the hydrolysis of other enzymes and opening up a new way for the utilization of biomass. We found that the synergistic action of LPMO and endochitinase from Bacillus cereus group can significantly improve the conversion efficiency of chitin to chitooligosaccharide.