Kd and Bmax of [³H]-PK 11195 binding using homogenates prepared from cytokine-treated DBT cells.

DBT cells incubated for 18–24 hrs with either vehicle (basal), TNFα (5 ng/ml), IFNγ (100 Ui/ml), TNFα+IFNγ, or TGFβ2 (1 ng/ml). Cells were harvested, homogenates prepared and [3H]-PK 11195 Kd and Bmax determined as outlined in the materials and methods section. Values are mean±SEM of 12–15 measurements (i.e. four or five independent experiments, each performed in duplicate). We found no significant difference in Kd and Bmax between basal and respective cytokine-treated conditions, as determined by ANOVA followed by Dunnett's.