Arabidopsis CRL interacts with OEP80 to facilitate its complex formation

Purpose: we conducted a froward genetic screen to isolate suppressors of the chloroplast-deficient crl mutant, and isolated a mutation affecting the OEP80 protein. Methods: We used EMS mutagenesis to find supressors of the crl mutant. Suppressors were phenotyped at the cellular and molecular (transcriptome) level, and OEP80 complex formation was investigated in the WT and crl mutant background. Results: a mutation in OEP80 fully restores the phenotype of the crl mutant, at the whole plant level, as well as at the cellular or transcriptome level Overall design: 3 biological replicates of RNA-seq from plantlets of WT, crl, and crloep80 suppressor mutants