Identification of ciliary protein interactome during senescence

Despite the importance of cellular senescence in human health, how damaged cells undergo senescence remains vague. We have previously shown that the translocation of a ciliary protein FBF1 to nucleus is essential for senescence induction in stressed cells. However, how dose FBF1 translocate from ciliary base to the nucleus still unclear. We employed BioID analysis to identify the potential interactor of FBF1 and associated protein that regulate FBF1 translocation. We discovered the ciliary base protein, CENEXIN1(ODF2), as interactor by using FBF1 as prey. We also explored CENEXIN1 (ODF2)interactome by APEX2-based BioID to idenfity KIFC3, a minus-end-directed kinesin, as downstream player that regulate the ciliary protein translocation.