Cytokines and PGE2 release in oxaliplatin-treated cells after acetaminophen, ibuprofen or N-acetyl cysteine treatment.

SH-SY5Y cells and RAW 264.7 cells were exposed 24 h to oxaliplatin (OXA) (200 µM) with a 30-min pre-treatment either with acetaminophen (AAP, 50 µM), ibuprofen (IBU, 1 µM) or N-acetyl cysteine (NAC, 1 mM). SH-SY5Y cell supernatant was collected and TNF-α, IL-1β and IL-6 levels (pg/mL) were assessed using ELISA kits according to the manufacturer's instructions. Prostaglandin E2 (PGE2) levels were determined in RAW 264.7 supernatant using a PGE2 Enzyme-Immuno-Assay kit. Values are the mean ± S.E.M. levels in pg/ml, five different assays per group. Significance of differences: OXA alone versus control, *p<0.05; AAP, or IBU, or NAC versus OXA alone: $ p<0.05.