Measure Celluar Force Using DNA-based Tension Probes: From Ensemble to Single-Molecule Studies - Source Data.

The source data of a protocol for the use of two advanced DNA-based tension probes, the reversible shearing DNA-based tension probe (RSDTP) and ForceChrono probe, which provide powerful tools for studying mechanotransduction in living cells.·This protocol describes the use of two advanced DNA-based tension probes, the reversible shearing DNA-based tension probe (RSDTP) and ForceChrono probe, which provide powerful tools for studying mechanotransduction in living cells.Compare to other probes, RSDTP enables reversible and repeatable measurements to be made across a wide range of forces, while ForceChrono probes capture force duration and loading rate, providing essential insights into the temporal dynamics of single-molecule force transmission.