Tissue cell counts and frequencies in SU5416 and RU486 treated micea.

aMice were treated with SU5416 (25 mg/kg/day) or equivalent amounts of vehicle. Some SU5416-treated mice were also treated with RU486 (50 mg/kg/day). Tissues were harvested after 3 days and labeled for flow cytometric analysis. Spleen and PLN were labeled for detection of CD4, CD8, and CD19 (B cells). Thymus was labeled for CD4 and CD8. Bone marrow was labeled for B220, IgM, and IgD. *Differences between vehicle and inhibitor-treated tissues were significant; p†p = 0.052 vs. vehicle control.bAbbreviations used: DP, CD4 and CD8 double-positive; BM, bone marrow; PLN, peripheral lymph node; Pro/Pre, IgM-IgD– progenitor/precursor B cells; Imm, IgM+IgD– immature B cells; Mature, IgM+IgD+ mature B cells.cFrequencies and total cell number of B cell populations in BM were calculated by gating on B220+ cells.