Bulk RNA-sequencing analysis of E17.5 DA from Prdm6f/f; SM22-Cre mice and wildtype littermates

RNA-sequencing analysis of E17.5 DA from Prdm6f/f; SM22-Cre mice and wildtype littermates identified contractile proteins such as Tagln and Myh11 as the most downregulated genes in the DA of Prdm6f/f; SM22-Cre mice vs. wildtype littermates from 280 differentially exressed genes. Strikingly, the expression levels of the Tfap2b and Sox9, which also function as NCC specifiers were significantly reduced at in DA of Prdm6f/f; SM22-Cre vs. wildtype littermates RNA-sequencing analysis of E17.5 DA from WT and Prdm6f/f; SM22-Cre mice.对来自Prdm6条件性敲除（Prdm6f/f）; SM22-Cre小鼠及其同窝野生型对照小鼠的胚胎第17.5天（E17.5）背主动脉（DA）开展RNA测序分析，在280个差异表达基因中，相较于同窝野生型小鼠，Prdm6f/f; SM22-Cre小鼠背主动脉内的转凝蛋白（Tagln）、肌球蛋白重链11（Myh11）等收缩蛋白编码基因呈现最为显著的下调趋势。值得注意的是，同样作为神经嵴细胞（Neural Crest Cells, NCC）特化因子的转录因子AP-2β（Tfap2b）与SRY-box转录因子9（Sox9），其在Prdm6f/f; SM22-Cre小鼠背主动脉中的表达水平同样显著降低。