Next generation sequencing of expression and differentially expression of miRNAs, lncRNAs and circRNAs in gastric adenocarcinoma

To systematically elaborate the transcriptomic landscape and complicated mechanism of gastric adenocarcinoma, we performed whole-transcriptome sequencing to screen miRNA, lncRNA, circRNA and mRNA expression profiles and the key functional ncRNAs were annotated. Human gastric cancer tissues and their corresponding non-tumorous adjacent tissues (≥5cm from cancer center) were collected from five patients who underwent gastrectomy at the Second Department of Surgical Oncology of General Hospital of Ningxia Medical University. Whole-transcriptome libraries were pooled and sequenced in a single lane of Illumina NovaSeq 6000 sequencing platform.