The 24 tryptic fragments of 60 kDa band determined by MALDI-TOF-MS analysis.

The tryptic fragments matched the N-terminal portion of human erythrocytic α spectrin as compared to the protein sequences of the NCBI sequence database. The identification was confirmed by complete de novo sequencing of two fragments (shown in Figure 3 B–C). Mass [M+H]+ denotes the mono-isotopic masses of the fragment ions; sequence range refers to the alignment of the sequence of the denoted fragments with the α-spectrin reference sequence (gi: 119573202); deviation from theoretical mass is the mass difference between the measured mass and the mass calculated from the corresponding database sequence; missed cleavage refers to the missed trypsin cleavage sites in the identified fragment; sequence is the fragment sequence in one-letter code, Mox is oxidized methionine.