Ethiopia SmartChip results: antibiotic resistance genes in anaerobic digestion

This dataset shows the cleaned data from Takara SmartChip PCR processing to quantify antibiotic resistance genes. The DNA samples were taken from six household-scale anaerobic digesters situated in Bishoftu, Ethiopia in 2020. Six samples of substrate (cow dung and water 1:1 mix) were taken on Day 1 of the study and six diegstate samples were taken on Day 36. As the digesters have a retention time of approximetly 36 days, this is representative of the effects of anaerobic digestion on the input substrate on Day 1. Thereofore the six substrate samples can be compared to the six digestate samples in terms of the relative abundance of antibiotic resistance genes and mobile genetic elements.

本数据集展示了来自Takara SmartChip PCR处理过程的清洁数据，用于量化抗生素抗性基因。2020年，从埃塞俄比亚比绍夫图地区的六座家庭规模厌氧消化器中采集了DNA样本。研究的第一天采集了六份底物样本（牛粪与水的1:1混合物），在第36天采集了六份消化状态样本。鉴于消化器的大约36天停留时间，这代表了厌氧消化对第一天输入底物的影响。因此，六份底物样本可以从抗生素抗性基因和可移动遗传元件的相对丰度方面与六份消化状态样本进行比较。