Figure 3 - Western Blots Raw Data

Raw image files to: <br><br>Figure 3: Western blot of fractionated P. falciparum-infected iRBCs. A) Pf2004 or B) Pf3D7 iRBCs at 10, 22, 34 and 46 hours post invasion (hpi) were separated via saponin lysis into into erythrocyte cytoplasm and PV content (RBC) and parasite cytoplasm and membranes (parasite). Western blot analysis was performed on total protein (left panel, 20 % loaded), saponin supernatant containing RBC and PV cytoplasm (middle panel, 20 % loaded) and pellet containing parasite cytoplasm (right panel, 100 % loaded). Antibodies targeting the RBC housekeeper hHsp70 (expected size human Hsp70: 70 kDa) and the parasite housekeeper PfBIP served as control for proper fractionation. Using two Ago2 antibodies, Ago2 was only detected in the total and the RBC fraction (expected size Ago2 95 kDa).