Figure 4

<b>PDF file: </b>Uncropped blots used in final figures for PSD95, synaptophysin and actin loading control in 5xFAD and TgA-5xFAD male mice 3-5 months old. <b>Figure 4 </b>Sheet 1<b> </b>(<b>b</b>)<b>: </b>Silver staining (20 x magnification) quantification of number of silver particles (analyzed from thresholded image) across the whole hippocampus 5xFAD and TgA-5xFAD mice. Three-four sagittal sections from at least four 5xFAD or TgA-5xFAD mice were used for all staining and immunostaining experiments. <b>Figure 4 </b>Sheet 2 and 3 (<b>d </b>and <b>e</b>)<b>:</b> Raw densitometric quantifications of synaptic markers PSD95 (sheet 1) and synaptophysin (sheet 2) in cortical lysates from 3-5-month old 5xFAD and TgA-5xFAD male mice densitometric quantification normalized to actin loading control. <b>Figure 4 </b>Sheet 4 (<b>g</b>)<b>: </b>Raw data for percent area quantification of cleaved caspase-3 immunofluorescence percent area across the hippocampus. <b>Figure 4 </b>Sheet 5 and Sheet 6: <b>h</b> Percentage time spent in each quadrant searching for removed escape platform in the Morris water maze in the probe trial (after 4 days of training to find an escape platform). Mice were given 60 s to explore the pool. Two days following the Probe trial, animals were put on the “Reversal” phase of the task, in which the target platform was placed in a different quadrant. Animals were trained for four days then memory was probed again and is denoted as the <b>i </b>Reversal probe. T= Target, O= quadrant Opposite to target quadrant, L= quadrant to the Left of the target quadrant, R= quadrant to the Right of the target quadrant.