Duplication analysis of novel activation loop motif of fungal MAPKs.

The most common activation loop motifs T-E-Y, T-G-Y, and K-G-Y of fungal MAPKs have Z-score of 10.86 with 100% confidence level, and are highly duplicated MAPKs. The MAPKs that contain the activation loop motifs T-T-Y, T-I-Y, S-E-Y, T-H-Y and T-N-Y, have a Z-score below four. Genes with Z-score of four or less were not considered as significant enough to be duplicated. Hence, MAPKs that contain Z-score below four are non duplicated MPAKs.