MiRNA degradation by NS3 of JEV [miRNA]

In order to further determine the je virus (JEV) infection in C57BL / 6 mice after its miRNA levels change, we use the Agilent micrornas chip as a platform, to detect the uninfected group (control group) and the experimental mouse total RNA, it is found that the miRNA levels decrease infection group, and picked out the ten changes obviously micrornas verification, the result is the same. Four week old healthy C57BL / 6 mice were selected. One group was injected with JEV virus as the experimental group. A group of injection medium was used as a control group. After the experimental group showed the corresponding symptoms, the two groups of mice were sacrificed immediately, the brain tissue was separated from the liquid nitrogen and sent to the company for detection of miRNA levels.