Krüppel-like factor 5 rewires NANOG regulatory network to activate human morulablastocyst specific LTR7Y and promote naïve pluripotency [RNA-seq]

Transcriptome profiling for KLF4 and/or KLF5 knockout lines in human naïve embryonic stem cells Transcriptome profiling for CRISPRa-LTR7Y or KLF5 overexpression in primed human embryonic stem cells KLF4 and/or KLF5 was knocked out by CRISPR-CAS9 in human primed embryonic stem cells; cells were adapted to naïve culture condition, and profiled for their transcriptome in comparison with wild type naïve and primed state embryonic stem cells The control primed hESCs and primed hESCs overexpressing CRISPRa-LTR7Y or pLVTH-KLF5 were collected for RNA-seq