Metastatic medulloblastoma remodels the local leptomeningeal microenvironment to promote further metastatic colonization and growth. Metastatic medulloblastoma remodels the local leptomeningeal microenvironment to promote further metastatic colonization and growth

Medulloblastoma metastasis often occurs in the leptomeningeal spaces; however, the biology of the metastatic tumour cells and the local leptomeningeal niche are poorly characterized. We performed single-cell RNA sequencing to analyze the leptomeningeal metastatic niche in a medulloblastoma mouse model. Overall design: Mouse SHH primary tumours and matched spinal leptomeninges with metastasis (4 mice) and normal spinal leptomeninges (3 control mice) were processed for single-cell RNA sequencing.