Zebrafish BBB transcriptome

To unravel novel genes and pathways involved in barriergenesis a transcriptomic dataset of 3 dpf and adult brain vasculature was generated by isolating the brain endothelium exploiting the selective uptake of the cation DAPI by the central nervous system vasculature. two condition experiments: 3dpf DAPI positive vascular cells versus peripherial vasculature, adult brain versus liver vasculature, 3dpf wild type vasculature versus peripheral tissue, and 3dpf gpr124 mutant versus wild type vasculature. biological replicates: 2. In brief: After DAPI exposure, Tg(kdrl:GFP) embryos were enzymatically and mechanically digested to generate a single cell suspension, followed by fluorescent activated cell sorting (FACS). RNA was processed and hybridized to Agilent zebrafish (V3) 4x44K to perform dual-color Microarray gene expression analysis (MOgene).