RNA-Seq data from U2 small nuclear RNA auxiliary factor 1 (U2AF1)-S34F and WT SKM-1 cells

Mutations in the U2 small nuclear RNA auxiliary factor 1 (U2AF1) gene are the common feature of a major subset in myelodysplastic syndromes (MDS). However, the genetic landscape and molecular pathogenesis of oncogenic U2AF1 S34F mutation in MDS are not totally understood. To understand how the cancer-associated U2AF1 S34F mutation promotes MDS, we next compared independent RNA samples of SKM-1 cells expressing wild-type U2AF1 and S34F mutant U2AF1 using RNA-sequencing (RNA-seq).Differentially expressed genes (DEGs) and significantly enriched pathways were identified by RNA-seq. Overall design: We constructed a recombinant lentivirus with FLAG-tagged wild-type or U2AF1 (S34F) mutant and stably transfected SKM-1 cells. Cells were administered the vehicle control and S34F mutants in triplicate, and total RNA was extracted with TRIzol reagent following the vendor's process. RNA sequencing was performed on the Illumina X10 system of LC Sciences (USA).