RNA-seq Analysis of the gene expression regulated by CID755673 in mouse embryonic stem cells

Purpose:The goals of this study is to analyze the candidate genes motified by CID755674 to promote the mouse embryonic stem cell (ESC) self-renewal through RNA-seq approach. Previously, we found that MEK inhbitior PD0323901 (PD) coporates with protein kinase D inhibitor CID755673 (CID) is able to maitain mouse embryonic stem cell (mESC) self-renewal, we named this conditon is "PC". In order to find out why CID755673 can promote mouse embryonic stem cell self-renewal, we performed RNA-sequence in mESCs treated with PD or PC, and tried to identify the key mechanisms under CID 46C mouse ESCs cultured in 1 µM PD0325901/serum condition in the presence or absence of CID755673 (named PD vs PC) for 24 hr and then were collected by using Trizol. Two repeat groups were set for each treatment group. These samples were sent to GENERGY BIO (Shanghai, China) company to do deep sequencing by using illumina Hiseq.