Quantitative analysis of colocalization of sumo1 and mutant lamin A and C.

Quantitative colocalization analysis of sumo1 and lamin A and C was performed using the ImageJ- 1.46r software. Thresholded Manders coefficients tM1 and tM2 and the percentage of pixels intensity above threshold colocalized (% Ch1 or Ch2 int > thresh) in each of the two channels (Ch1 for sumo (green fluorescence) and Ch2 for lamin A and C (red fluorescence) were calculated using the threshold algorithm of Costes et al (2004) [43].