Pyrosequencing reliably quantified the methylation of four consecutive CpG dinucleotides per individual (chr2:100721843–100721885; hg19).

A fifth CpG site is preceded by a 7 base pair T stretch (after bisulfite conversion), making it impossible to assess the exact percentage of cytosines compared to thymines for that particular site due to the limitations of the pyrosequencing technique. For each individual at least three separate analysis of different bisulfite conversions were performed. Average methylation percentages and confidence intervals are presented in this table. Methylation cut-off value was set at 10% according to the manufacturers guidelines.