Effect of constitutive AHR activation in skeletal muscle. Effect of constitutive AHR activation in skeletal muscle

To investigate the RNA differences in muscle with and without constitutive AHR activation. AAV9 was used to express either a green fluorescent protein (control) or mutant AHR protein that exhibits constitutive transcriptional activity Overall design: Male C57BL6J mice were infected with AAV9 viruses encoding a GFP or constitutively active AHR driven by the human skeletal actin promoter to cause myofiber-specific expression. AAV's were injected into the gastrocnemius mucle of 5-month old mice and muscles were harvested 5 months later for RNA isolation