Figure2. Plots showing specificity of fluorescent proteins co-expressed within 293T cells overlaid with single fluorophore expression data.
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To asses functional and bleed over properties between fluorescent proteins by coexpressing them Conclusion: Up to four different fluorescent proteins could be combined without compensatory issues. Optimally use of BFP2 with IFP/TagRFP657 and Apple/mCherry/Tomato and Venus/YFP should result in good separation characteristics. Avoid GFP. Experiments were repeated multiple times.
创建时间：
2018-03-01
