Stochastic 10-cell profiling of an MCF-10A clone expressing chimeric EGFR and Erbb2 receptors

ErbB2 activation of MCF10A cells gives rise to a multiacinar phenotype that is incompletely penetrant. To identify candidate upstream regulators of the phenotype, an MCF10A-5E clone was subcloned to express chimeric receptors of human EGFR and rat Erbb2, and this B2B1 subclone was stochastically profiled (PMID: 20228812) after 24 hours of 3D culture with or without synthetic dimerization. B2B1 cells were grown in 3D culture for six days, treated with or without synthetic heterodimerizer (AP21967) for 24 hours, and cryoembedded for frozen sectioning. 10-cell samples or pool-and-split controls were randomly collected by laser-capture microdissection for analysis of transcriptional regulatory heterogeneities by stochastic profiling (Janes et al., Nat Methods 7(4):311-7 [2010]).