Single-cell transcriptomic analysis of aged mouse subcutaneous adipose tissue

Adipose tissue (AT) is an essential endocrine organ which undergoes significant changes during aging. Functions of vascular endothelial cells are altered in aged AT, and markedly affect the metabolic homeostasis of AT. However, little is known about the aged alterations of EC in AT, and whether and how their metabolic changes contribute to AT aging. Stromal vascular fraction (SVF) cells in inguinal subcutaneous adipose tissues (SAT) were isolated from young (3-months-old, n=4) and old (18-months-old, n=3) mice and subjected to single-cell RNA sequencing (scRNA-seq) using 10x Genomics platform.脂肪组织（Adipose Tissue, AT）是一类不可或缺的内分泌器官，在衰老进程中会发生显著改变。衰老的脂肪组织内血管内皮细胞的功能出现异常，这会显著影响脂肪组织的代谢稳态。然而，目前学界对脂肪组织中血管内皮细胞（endothelial cell, EC）的衰老相关改变尚不清楚，也未明确其代谢变化是否以及如何参与脂肪组织的衰老过程。本研究从年轻（3月龄，n=4）与老年（18月龄，n=3）小鼠的腹股沟皮下脂肪组织（Inguinal Subcutaneous Adipose Tissue, SAT）中分离得到基质血管组分（Stromal Vascular Fraction, SVF）细胞，并采用10x Genomics平台对其开展单细胞RNA测序（single-cell RNA sequencing, scRNA-seq）。