Pyruvate kinase splice variants in fibroblasts influence cardiac remodeling after myocardial infarction in male mice

Fibroblasts are crucial for cardiac repair after myocardial infarction (MI). In response to signaling cues, they differentiate to phenotypes with robust capacities to synthesize and secrete extracellular matrix (ECM) and signaling molecules. Although activated fibroblast phenotypes are associated with pronounced changes in metabolism, it remains unclear how the metabolic network upholds the effector functions of fibroblasts in the infarcted heart.  We found that two enzymes that could facilitate a phosphoenolpyruvate cycle, i.e. pyruvate kinase muscle isoform 2 (PKM2) and phosphoenolpyruvate carboxykinase 2 (PCK2), are elevated in the heart after MI. Although Pck2 deletion had no effect on post-MI remodeling, fibroblast-specific switching of Pkm2 to Pkm1 (fbPkm2→1) mitigated ventricular dilation, wall thinning, and losses in ejection fraction caused by MI. Despite these salutary effects, fbPkm2→1 switching did not alter cardiac fibrosis in vivo, nor did it affect collagen productio..., , # Pyruvate kinase splice variants in fibroblasts influence cardiac remodeling after myocardial infarction in male mice

Dataset DOI: [10.5061/dryad.tx95x6b8w](10.5061/dryad.tx95x6b8w)

## Description of the data and file structure

The data included in this submission involve multiple data types, including data related to metabolism, gene expression, protein abundance and secretion, and cardiac function.  The uploaded data include the raw data used to generate the graphs, with as little redundancy with the published manuscript as possible.  In addition, RNA seq data were generated, which are available NCBI GEO database under Accession No. GSE291984.

## Files and variables

### File: Figure_1.csv

Description: PKM2 abundance increases in the infarcted heart and is found in areas of replacement fibrosis.

Immunoblots and relative quantification of PKM1 and PKM2 in whole heart homogenates 5 d after sham or MI surgery. n = 8 male mice per group. Proteins were normalized to amido black sign..., ,