Map active enhancer and gene promoter regions in the SCN

Histone ChIP-seq was performed at varied time of the day to map the gene promoter and active enhancer sites in the central clock; SCN (suprachiasmatic nuclei). Chromatin immunoprecipitation DNA-sequencing (ChIP-seq) for the histone modifications H3K4me3 and H3K27ac in adult mouse SCN and cortex at distinct time of the day . WT C57Bl/6J mice were used for SCN tissue collection at 6 distinct time-points starting from ZT3 at every 4 hours, where lights on at 7 am (ZT0) and lights off at 7 pm (ZT12). We also collected cortical punches at ZT3 and ZT15 to compare and establish SCN enriched chromatin modifications. For histone chromatin immunoprecipitation two separate biological replicates per time-point per tissue-type was collected where each biological replicate composed of 3-4 individual SCN or cortical punches.