Oligonucleotides used as template.
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Single-stranded oligonucleotides containing the wild-type and modified sequences of the φ29 or Nf DNA right replication origin were obtained from Sigma. These oligonucleotides were used as template for both the initiation assay (TP-dNMP formation) and the truncated elongation assay. THF indicates the presence of tetrahydrofuran, which is a stable analogue of an abasic site.
创建时间：
2015-12-02
