Transcriptomic analysis of Organs Following Stem Cell Therapy for Dengue (Denv 2) Infection

Dengue virus can infect and cause systemic damage in many organs, resulting in organ failure. Here, we present a novel report showing a tailored stem cell-based therapy which can aid in viral clearance and rescue liver and blood vessels cells from further damage during dengue infection by administering combination of hematopoietic stem cells, endothelial progenitor cells and dental pulp stem cells in a dengue virus-infected BALB/c mouse model. To analyze the molecular level changes induced by DENV infection and stem cell treatment in dengue-infected mice, RNA sequencing (RNA-seq) using MiSeq next-generation sequencing was employed in this study. Transcriptomics analysis was performed comparing gene expression patterns in different experimental groups. Methods: Liver mRNA profiles of 21-day-old healthy, Dengue virus infected, and dengue infected plus stem cells treated BALB/C mice were generated by sequencing, in triplicate, using Illumina Miseq V3 150. The sequence reads that passed quality filters were assembled and analyzed using Tuxedo software. The results were analyzed using Cuffdiff to identify differentially expressed genes and transcripts. The relative expression of genes was determined based on FPKM (total fragments per kilobase of exon per million mapped reads) values Results: After analysis, it was found that 59 genes were significantly up-regulated in the DVI group, and in the DVI-SCT group, 47 of the genes were successfully corrected to the level similar to that of control group, while the remaining genes showed down-regulation Conclusion: In the recent years, the incidence of dengue has continued to increase, but there is currently no rapid and effective treatment available. This study demonstrates stem cell therapy as a promising management to reduce the global burden of dengue. Overall design: Liver and blood vessels mRNA profiles of 21-day-old healthy, Dengue virus infected, and dengue infected plus stem cells treated BALB/C mice were generated by sequencing, in triplicate, using Illumina Miseq V3 150.