Transcriptional profiling of human serotonergic neurons in vitro

"Human serotonergic neurons are derived using published transdifferentiation protocols. ""Human Fibroblasts"" correspond to human fibroblasts (from Line#1, Coriell bought (AG08498)). Samples labeled ""human neurons or induced neurons (iN)"" correspond to neurons transdifferentiated from fibroblasts using two transcription factors, as previously described (#1-AG08498 or #2:ERF-1, Erlangen Germany, a line given to us by collaborators) into primarily glutamatergic neruons. Samples labled ""5-HT neurons or serotonergic neurons or iSN"" correspond to serotonergic neurons derived from the stated fibroblast lines, using an additional four transcription factors. For transdifferentiation of iN and iSN, fibroblasts were made to overexpress the stated transcription factors in a doxycycline inducible manner for up to 3 weeks, and then neurons are sorted out and collected directly into Trizol for RNA preparation and sequencing. The non-transdifferentiated fibroblast lines were collected in bulk withtout differentiation into neurons. The line number corresponds to the same fibroblast line either being transdifferentiated into iN or iSN, as labeled - for direct and groupwise comparison."