The role of alternative 3UTR polyadenylation in adaptation to ethanol in Drosophila

These files are the original fastq files used in the The role of of alternative 3 prime polyadenylation in adaptation to ethanol in DrosophilaAlternative mRNA processing, such as splicing and alternative polyadenylation, have been demonstrated to be important for macro-evolutionary trends such as organismal complexity. For example,the number of coding genes and the length of their coding sequence has remained relatively stable between worms and human. However, the number of genes that produce alternative 3 UTRs has doubled, and the length of those UTRs has increased 8.5x. Alternative 3 UTRs mediate cis-regulatory interactions with other proteins which affects stability, localization, and translational efficiency. This is important for cell-type specific gene expression and organismal development.Given that ethanol is a common environmental stressor that Drosophila encounters frequently and processes such as alternative polyadenylation are good candidates for the stress response, we wanted to investigate changes in alternative polyadenylation upon ethanol exposure in flies that were or were not adapted to ethanol. We sequenced the polyadenylation sites in the 3 UTRs of flies from French and Zambian Drosophila both with and without being exposed to ethanol.