A targeted gene panel for circulating tumor DNA sequencing in neuroblastoma

We report the development of a targeted Next Generation Sequencing (NGS) gene panel for ctDNA sequencing that is tailored to the genetic landscape of neuroblastoma. The ctDNA detection and sequencing, through an ad hoc designed gene panel, could complement or replace tissue biopsies necessary for diagnosis of neuroblastoma. The application of the suggested strategy for the diagnosis of neuroblastoma, may improve disease stratification at diagnosis and provide useful information for therapy decisions making.