The bone marrow endothelial progenitor cell response to septic infection: Dataset

An early increase in the level of endothelial progenitor cells (EPCs) in the systemic circulation occurs in patients with septic infection/sepsis. The significance and underlying mechanisms of this response remain unclear. This study investigated the bone marrow EPC response in adult mice with septic infection induced by intravenous injection (i.v.) of Escherichia coli. For in vitro experiments, sorted marrow stem/progenitor cells (SPCs) including lineage(lin)-stem cell growth factor receptor(c-kit)+stem cell antigen-1(Sca-1)-, lin-c-kit+, and lin- cells were cultured with or without lipopolysaccharides (LPS) and recombinant murine vascular endothelial growth factor (VEGF) in the absence and presence of anti-Sca-1 crosslinking antibodies. In a separate set of experiments, marrow lin-c-kit+ cells from green fluorescence protein (GFP)+ mice, i.v. challenged with heat-inactivated E. coli or saline for 24 h were subcutaneously implanted in Matrigel plugs for 5 weeks. Marrow lin-c-kit+ cells..., Animals
C57BL/6 and BALB/c mice (6-8 weeks old, both sexes) were purchased from Charles River Laboratories (Wilmington, MA) and Taconic Biosciences, Inc. (Germantown, NY). Breeding pairs of C57BL/6-Tg (UBC-GFP)30Scha/j mice were purchased from the Jackson Laboratory (Bar Harbor, ME). C57BL/6-Tg (UBC-GFP)30Scha/j mice and Sca-1-/- mice (Sca-1 knockout or Sca-1 KO considered congenic on the C57BL/6 background, originally transferred from Dr. William L. Stanford’s group at University of Toronto, Toronto, Canada) were bred under specific pathogen-free conditions in the Animal Care Facilities of Northeast Ohio Medical University, Michigan State University, and Louisiana State University Health Sciences Center, respectively. These mice were used for experiments at the age of 6-8 weeks old. All animals were housed in specific pathogen-free facilities with a 12-hour light/dark cycle. Approvals from the Institutional Animal Care and Use Committees in adherence with National Institutes of Health ..., , # The bone marrow endothelial progenitor cell response to septic infection: Dataset

## Author Information

1. A. Principal Investigator Contact Information
Name: Ping Zhang
Institution: Northeast Ohio Medical University
Address: Rootstown, OH USA
Email: [[e-mail]](mailto:[e-mail])

B. Associate or Co-investigator Contact Information
Name: Xin Shi
Institution: Northeast Ohio Medical University
Address: Rootstown, OH USA
Email: [[e-mail]](mailto:[e-mail])

Name: Kevin J. Simms
Institution: Northeast Ohio Medical University
Address: Rootstown, OH USA
Email: [[e-mail]](mailto:[e-mail])

Name: Thomas J. Ewing
Institution: West Clinical Laboratory, Lakeland Regional Health Medical Center
Address: Lakeland, FL USA
Email:[[e-mail]](mailto:[e-mail])

Name: Yuan-Ping Lin
Institution: 3R Life Sciences Ltd.
Address: Kaohsiung City, Taiwan
Email: [be...