Next Generation Sequencing Analysis of human pluripotent stem cells derived endothelial and smooth muscle cells. Next Generation Sequencing Analysis of human pluripotent stem cells derived endothelial and smooth muscle cells

Purpose: global gene expression profiling of H1 and iPSC derived endothelial cells (ECs) and smooth mucle cells (SMCs). Methods: SMART-seq2 amplified polyA RNA from undifferentiated H1 cells and iPSCs (in biological duplicates), cardiovascular progenitor cells, endothelial cells (ECs) and smooth muscle cells (SMCs) Results: Insulin free condition promoted cardiovascular mesoderm, endothelial and smooth muscle differentiation. Conclusions: the gene expression profiles of ECs and SMCs differentiated in insulin free medium confirmed that they are true ECs and SMCs. Overall design: Perform RNA-seq of undifferentaited H1 and human iPSCs, day 3 mesoderm progenitor cells, day 8 endothelial and smooth muscle cells.