Electrical parameters (mean±SEM) of LS513, HT29 MTX-P8 and HT29 MTX-E12 intestinal cell lines.

Electrical parameters were measured in Ussing chambers for the LS513, HT29 MTX-P8 and HT29 MTX-E12 intestinal cell line cultured on snapwell membranes for 21 days post confluency. Under standard conditions the cells were immersed in media. Under semi-wet interface with mechanical stimulation conditions (SWMS), 50 µl of media was applied on the apical side whereas the basolateral side was immersed in media. 10 µM of DAPT was added basolaterally during the first 6 days of semi-wet interface to the DAPT treated cells. Data are presented as mean ± standard deviation.
*, **respectively indicates p<0.05 and p<0.01 compared to same cell line cultured under standard conditions, Anova, dunnetts post hoc.