Population growth rate data and scripts concerning the publication:"Redundancy and the role of protein copy numbers in the cell polarization machinery of budding yeast"experiments

The data entails raw and processed data from population growth rates experiments using different yeast mutants. Moreover, it contains the required scripts to processthe data and reproduce Fig3 and Supplemental Figures 4 and 5 from the paper "Redundancy and the role of protein copy numbers in the cell polarization machinery of budding yeast" published in Nature Communications.The experiments investigated the role of Cdc42 copy number in the polarization efficiency of different relevant mutants containing knockout mutations in Bem1 and Bem3 genes. The Cdc42 copy number was controlled using a galactose promoter.Initially the growth measurements were taken using a sfGFP tag fussed in Cdc42 (data shown in Fig3). Therefore, we did control experiments, shown in the Supplemental material, to check that the sfGFP tag was not influencing the results we observed previously (Supp Fig 4 and Supp Fig 5).<br><br><br>

本数据集涵盖使用不同酵母突变体开展种群生长速率实验所获得的原始数据与经处理后的数据。此外，数据集还附带了用于处理数据、复现发表于《自然-通讯（Nature Communications）》的论文《Redundancy and the role of protein copy numbers in the cell polarization machinery of budding yeast》中的图3及补充图4、5的配套脚本。

本实验探究了Cdc42拷贝数在携带Bem1与Bem3基因敲除突变的相关突变体的细胞极化效率中的调控作用。研究中通过半乳糖启动子调控Cdc42的拷贝数。最初，实验通过在Cdc42上融合超折叠绿色荧光蛋白（sfGFP, superfolder Green Fluorescent Protein）标签完成生长量测定，相关数据展示于图3。为此，我们开展了详见补充材料的对照实验，以验证sfGFP标签不会对此前观测到的实验结果造成干扰，该实验对应补充图4与补充图5。