Expression of Cullin and F-box proteins by microarray in isolated cardiomyocytes and fibroblast

Microarray expression data from C57/Bl6Nand b1-adrenergic transgenic mice, including heart tissue, isolated cardiomycytes and fibroblast, and lung fibroblasts Expression data was further used to identify promising targets in cardiomyocytes that are also members of the ubiquitin proteasome system that are upregulated in heart cells This analysis aimed to evaluate the expression level of member of Cullin-RING ligases in the heart. Cullin-RING complexes (CRLs) constitute the largest group of E3 ligases, which are characterized by two signature components: a cullin (CUL) scaffold protein and the RING (for Really Interesting New Gene) finger protein ROC1. In the prototypic SCF (Skp1•CUL1•F-box protein•ROC1) complex, the CUL1 N-terminus binds to the Skp1•F-box protein substrate-targeting module, whereas the C-terminally located cullin domain anchors ROC1, which recruits E2 conjugating enzyme to catalyze the transfer of Ub to the substrate protein. SCF contains a substrate recognition subunit known as the F-box protein, characterized by a 40-amino-acid F-box domain (Skaar et al., 2013). F-box proteins are interchangeable components of the SCF type E3 ligases and dictate the ubiquitin ligase's substrate specificity. Of the 69 F-box proteins encoded by the human genome, only a fraction has been studied in the heart. We harvested mice hearts, isolated cardiomyocytes, fibroblasts, and lung fibroblasts to evaluate the expression levels of CRL members We analyzed samples from 5 mice pooled into each sample using the Affymetrix Mouse Expression Set 430. Array data was processed by Affymetrix Exon Array Computational Tool. No techinical replicates were performed. The file Cul Aros array depicts the microarray expression of the Cullin scaffold protein, while the expression data of the various F-box proteins are shown in Fbx Aros array sheet. These two excel sheets depict the expression level in a Log2 scale of the respective samples. *** Submitter declares the CEL files are no longer available. ***