Detection of Multiple Fish Species Using Environmental DNA (eDNA), Alaska 2018

This release provides results of an environmental DNA (eDNA) study of fish identified in seven Alaskan lakes, three streams, and a positive control. Samples included replicates for multiple sampling dates and locations. The reference list and conserved primer sets to identify species present were developed using publicly available data (https://www.ncbi.nlm.nih.gov/genbank/). Highly conserved primers which could differentiate species of interest were developed for three portions of mtDNA genes (12S, 16S and COI).