Transcriptome sequencing in Scutiger sikimmensis

Data in Brief
Title: Transcriptome sequencing and the presence of high-altitude and sex-determination genes in Scutiger sikimmensis
Authors: Sylvia Hofmann1, Heiner Kuhl2, Chitra Bahadur Baniya3, Matthias Stöck2

Affiliations
1UFZ - Helmholtz-Centre for Environmental Research, Department of Conservation Biology, Permoserstrasse 15, 04318 Leipzig, Germany
2Leibniz-Institute of Freshwater Ecology and Inland Fisheries, Müggelseedamm 301, 12587 Berlin, Germany
3Tribhuvan University, Central Department of Botany, Kirtipur, Kathmandu, Nepal

Abstract
The Himalayas are one of earth’s hotspots of biodiversity. However, modern evolutionary genomic studies in Himalayan vertebrates are still at the beginning. Moreover, in organisms, like most amphibians with relatively high DNA content, whole genome sequencing remains bioinformatically challenging and no complete nuclear genomes are available for Himalayan amphibians. Here, we present the first well-annotated multi-tissue transcriptome of a Himalayan lazy toad (Scutiger sikkimensis). Applying Illumina NextSeq 500 RNAseq to six tissues, we obtained 41.32 Gb of sequences, assembled to ~111,000 unigenes, and annotated them in seven functional databases. The annotation results and their statistics can be found in Supplementary Material S8 and S9.

Key words: Himalayan lazy toad; Trinity; RNAseq; Illumina; N50
Specifications
Organism/cell line/tissue: Scutiger sikkimensis/brain, heart, liver, lung, kidney, testis tissue
Sex: male
Sequencer or array type: Illumina NextSeq 500
NCBI Project ID: PRJNA532534
Accession numbers: SAMN11403593-SAMN11403598SRA numbers: SRR9953599-SRR9953604
Experimental factors: De novo transcriptome assembly of Scutiger sikkimensis
Experimental features: Tissue samples of a male specimen of the lazy toad Scutiger sikkimensis was collected for total RNA isolations. Prepared a paired-end library, sequenced by the NextSeq 500 system. The obtained data was subjected to de novo assembly.
Consent: N/A
Sample source location: Central Himalaya, Nepal