DNA methylation of colorectal cancer stem cells. DNA methylation of colorectal cancer stem cells

Colorectal cancer stem cells (CR-CSCs) are implicated in tumor development, metastasis, and therapy resistance, however little is known about their epigenetic features. Here we describe the DNA methylation profiles of eight CR-CSC lines and their distinctive patterns, which are maintained following in vivo passages in immune-compromised mice. Overall design: DNA was bisulfite-converted using the EZDNA Methylation-Gold Kit (Zymo Research) and analysed using the Infinium HumanMethylationEPIC BeadChip (Illumina) following manufacturer's instructions.