Sequences RFP full length genes
收藏DataONE2014-12-11 更新2024-06-27 收录
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资源简介：
All raw sequences used to reconstruct the amilFP597 gene in A. millepora.Full-length sequences of indel (-) and indel (+) promoter variants of the amilFP597 gene were obtained for the MR morph. These sequences, extending from the promoter region to the 3’UTR, were produced by joining the
sequences of two overlapping PCR products covering the 5’ region [promoter to exon 3 amplified using primers
pRFPlargeF (+) or pRFPsmallF (-) and RFP SP1] and the 3’ region (intron 2 to 3’UTR amplified using primers
RFP_I2-3’U_F and RFP_I2-3’U_R2). Sequence differences in the overlap between the 5’ and 3’ region
fragments were used to assign the latter to either the indel (+) or indel (-) promoter variant genes. The
promoter-exon 3 and intron 2-3’UTR fragments were cloned and sequenced on both strands using vector
primers and by primer walking. The assembled sequences have been submitted to GenBank as accessions
KC818413 [indel (-) gene] and KC818414 [indel (+) gene].
创建时间：
2014-12-11
