Sampling details for target organism, <i>T</i>. <i>urticae</i> used in specificity tests of the real-time PCR assay.

All the samples were identified with morphological first and then confirmed by DNA sequences. Asterisk (*) denotes samples which could not be identified morphologically and were identified using COI and/or ITS sequences, a DNA extractions were performed using several methods, QG = Qiagen DNeasy Blood and Tissue kit; ZR = ZR Tissue & Insect DNA kit (Zymo Research, CA, USA); PG = PrepGEM (ZyGem, USA), KF = Kingfisher Cell and Tissue kit (Thermo Scientific, USA). b All samples were tested in duplicate and all Cq values provided are means of the duplicate wells. Sampling details for target organism, T. urticae used in specificity tests of the real-time PCR assay.