Expression data from E18.5 Igf1 -/- (homozygous mutant) and Igf1+/+ (normal wild type control) mouse lungs

Insight into the role of Insulin-like Growth Factor (IGF) in development of lungs has come from the study of genetically modified mice. IGF1 is a key factor during lung development. IGF1 deficiency in the neonatal mouse causes respiratory failure collapsed alveoli and altered alveolar septa. To further characterize IGF1 function during lung development we analyzed Igf1-/- mouse prenatal lungs in a C57Bl/6 genetic background. Mutant lungs showed disproportional hypoplasia, disorganized extracellular matrix and dilated alveolar capillaries. IGF1 target genes during lung maturation were identified by analyzing RNA differential expression in Igf1-/- lungs using microarrays. Lungs from E18.5 were isolated from both Igf1+/+ wild type and Igf1-/- null mice and pooled to obtain RNA. Heterozygous male and female with a genetic background C57BL/6J were mated to obtain embryos at embryonic (E) stage 18.5 days post coitum (E18.5). 3 biological replicates per genotype.胰岛素样生长因子（Insulin-like Growth Factor, IGF）在肺脏发育中的功能机制，可通过基因工程改造小鼠的相关研究得以阐明。IGF1是肺脏发育过程中的关键调控因子，新生小鼠的IGF1缺失会引发呼吸衰竭、肺泡塌陷及肺泡隔结构异常。为进一步明确IGF1在肺脏发育中的功能，本研究对C57Bl/6遗传背景下的Igf1-/-小鼠胎肺组织展开分析，结果显示突变型胎肺存在比例失调的发育不全、细胞外基质结构紊乱以及肺泡毛细血管扩张。本研究通过基因芯片技术分析Igf1-/-胎肺组织的RNA差异表达谱，鉴定出肺脏成熟过程中IGF1的靶基因。以遗传背景为C57BL/6J的杂合子雌雄小鼠进行交配，获取交配后胚胎发育至第18.5天（E18.5）的胚胎，于该时间点分别从Igf1+/+野生型小鼠与Igf1-/-纯合敲除小鼠体内分离胎肺组织，混合后提取总RNA，每种基因型设置3个生物学重复。