Identification of neural progenitor cells and their progeny reveals long distance migration in the developing octopus brain (Deryckere et al., 2021)

In order to construct a full-length transcriptome of Octopus vulgaris embryos and paralarval brains, the Iso-Seq method was used. RNA was extracted from a pool of 25 Stage XI-XII embryos and dissected brains of one-day-old paralarvae using Tri-reagent (Invitrogen) and the Qiagen Micro kit (Qiagen). Samples were sequenced on the PacBio Sequel at the Genomics Core at KU Leuven (Belgium) following the protocol recommended by PacBio. Only cDNAs containing polyA-tails were selected, with the aim to retrieve full-length transcripts.